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Cytofect™ Transfection Kits contain a new class of unique chemical reagent formulations specifically optimized for transfecting primary cells. The NucleofectorTM Technology, originally introduced into the market by legacy Amaxa in It enables highly efficient, transfection of primary cells, stem. Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus.

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Modern instrumentation allows nucleic acid delivery to the nucleus and successful transfer of DNA and RNA into primary and stem cells. Electroporation has. Primary human CD3+ cells were transfected through electroporation either with the Neon electroporator (Life Technologies), the Lonza 4D Nucleofector device. We have demonstrated that unprecedented transfection results are achieved with primary cells when novel electroporation conditions are combined with a treatment.

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We have demonstrated that unprecedented transfection results are achieved with primary cells when novel electroporation conditions are combined with a treatment. Effectene Transfection Reagent has been used for transfection of a variety of different cell lines and primary cells, and yields significantly better. Primary human CD3+ cells were transfected through electroporation either with the Neon electroporator (Life Technologies), the Lonza 4D Nucleofector device.